To further investigate the differential effects exerted by TD and TI-1 antigens on trout IgM+ B cells, RNA sequencing (RNA-seq) was performed on FACS isolated splenic IgM+ B cells that had been stimulated with TNP-KLH or TNP-LPS for 24 h, or on control unstimulated splenic IgM+ B cells Overall design: 9 samples 3 biological replicates per treatment including unstimulated IgM+ B-cell as control and TNP-KLH and TNP-LPS stimulated IgM+ B-cells