During the production of RNAi transgenic lines, we identified a gel-less mutant in the progeny of one T0 line out of nine (line L2). A S1 population of 114 plants segregating for the gel-less phenotype was generated by self-pollination of a heterozygous Micro-Tom gel-less mutant T2 plant (line L-2.10). WT-like and mutant-like bulks were constituted based on the gel-less phenotype for further whole genome sequencing. S1 offsprings were used to constitute the bulks. Equal amounts of leaf tissue from 45 S2 plants (descendant of five S1 plants) were pooled for the WT-like and from 27 S2 plants (descendant of five S1 plants) for the mutant-like bulk. The WT-like bulk was enriched for the homozygous WT allele by selecting S1 progenies that did not segregate for the gel-less phenotype. Genomic DNA was extracted as previously described (Garcia et al., 2016) and sequenced on an Illumina HiSeq 2000 sequencer operating in a 100-bp paired-end run mode at the INRA-GeT-PlaGe-GENOTOUL platform.