Two-year-old brood stock rainbow trout families were stripped in 2015 spring spawning season, and maintained in spring water in Troutlodge hatchery at Sumner, WA, USA. About 80 eggs from each family were collected and immediately frozen in liquid nitrogen, then shipped to NCCCWA in dry ice and then kept in -80°C freezer for RNA isolation. The remaining eggs were fertilized with freshly stripped semen. The fertilized eggs were incubated in the hatchery to evaluate survival rate at eyeing stage. The eggs from 20 female families with survival rates of 0 to 97.69% were selected for transcriptome analysis.