Library with the digested RAW data for the publication Imaging tissue-mimic with light sheet microscopy: A comparative guideline. This includes The cropped stacks used for the generation of the results presented in the publication. The dataset contains multitiff images of acquisitions corresponding to the different conditions studied. 5 Imaging conditions: Gaussian 1 photon, Gaussian 2 photon, Bessel 1 photon, Bessel 2 photon and Bessel 1 photon with confocal line. 4 type of samples: Neurospheres, Tumor Spheroids, Mamalian cell speroids and cardiac cells spheroid. 2 Sample conditions: Fluorescent labeling (SRB) and embedded Beads.