The data were obtained from peripheral blood samples from a cohort of 60 breast cancer patients in remission. The data included in this file have been generated with the purpose of evaluating radioinduced apoptosis and γ-H2AX phosphorylation. These two biomarkers have been described as potential biomarkers of radiotherapy toxicity. Additionally, an analysis of 27 SNPs associated with apoptosis is also included. The analysis of γ-H2AX was performed after ex vivo irradiation of lymphocytes at a dose of 2 Gy, and was analyzed after 1, 2, 4 and 24 h after irradiation. The analysis of apoptosis was carried out after irradiating the lymphocytes at 8 Gy and was analyzed after 24 and 48 hours. Both γ-H2AX and apoptosis have been analyzed by flow cytometry.

METHODOLOGICAL INFORMATION

1. Description of methods used for collection-generation of data Double strand breaks were evaluated by geometric means of γ-H2AX fluorescence in sham irradiated and after 1, 2, 4 and 24 h post-irradiation. Cell death was evaluated with the Annexin-V-FLUOS Staining Kit (Roche, Barcelona, Spain) and assessed by flow cytometry. For the SNP analysis, DNA extraction was done using the QIAamp DNA Blood Midi Kit (QIAGEN, Hilden, Germany). Genotyping assay was done using the Open Array Plate (Thermo-Fisher, Eugene, OR, USA).

2. Methods for processing the data: The file contains the data obtained for the sixty breast cancer patients in total remission. For Genotyping analysis R studio was used. To analyse the γ-H2AX assay and cell death, FlowJo vX.0.7 software was used.

3. Instrument- or software- specific information needed to interpret the data: CSV files can be opened in notepad or other text editors

4. Instruments, calibration and standards information: γ-H2AX and cell death was measured by flow citometry with CytoFLEX (Beckman Coulter Life Science, Pasadena, CA, USA).Genotyping assay was done using Open Array Plate (Thermo-Fisher, Eugene, OR, USA).