Temperature affects the morphology and calcification of Emiliania huxleyi strains


The global warming debate has sparked an unprecedented interest in temperature effects on coccolithophores. The calcification response to temperature changes reported in the literature, however, is ambiguous. The two main sources of this ambiguity are putatively differences in experimental setup and strain-specificity. In this study we therefore compare three strains isolated in the North Pacific under identical experimental conditions. Three strains of Emiliania huxleyi type A were grown under non-limiting nutrient and light conditions, at 10, 15, 20 and 25 ºC. All three strains displayed similar growth rate versus temperature relationships, with an optimum at 20-25 ºC. Elemental production (particulate inorganic carbon (PIC), particulate organic carbon (POC), total particulate nitrogen (TPN)), coccolith mass, coccolith size, and width of the tube elements cycle were positively correlated with temperature over the sub-optimum to optimum temperature range. The correlation between PIC production and coccolith mass/size supports the notion that coccolith mass can be used as a proxy for PIC production in sediment samples. Increasing PIC production was significantly positively correlated with the percentage of incomplete coccoliths in one strain only. Generally, coccoliths were heavier when PIC production was higher. This shows that incompleteness of coccoliths is not due to time shortage at high PIC production. Sub-optimal growth temperatures lead to an increase in the percentage of malformed coccoliths in a strain-specific fashion. Since in total only six strains have been tested thus far, it is presently difficult to say whether sub-optimal temperature is an important factor causing malformations in the field. The most important parameter in biogeochemical terms, the PIC:POC, shows a minimum at optimum growth temperature in all investigated strains. This clarifies the ambiguous picture featuring in the literature, i.e. discrepancies between PIC:POC-temperature relationships reported in different studies using different strains and different experimental setups. In summary, global warming might cause a decline in coccolithophore's PIC contribution to the rain ratio, as well as improved fitness in some genotypes due to less coccolith malformations.

The carbonate system was calculated from temperature, salinity (32), TA and DIC, using the the program CO2Sys (Lewis and Wallace, 1998), applying the equilibrium constants from Mehrbach et al. (1973), refitted by Dickson and Millero (1987).

Supplement to: Rosas-Navarro, Anaid; Langer, Gerald; Ziveri, Patrizia (2016): Temperature affects the morphology and calcification of Emiliania huxleyi strains. Biogeosciences, 13(10), 2913-2926

DOI https://doi.org/10.1594/PANGAEA.860214
Related Identifier IsSupplementTo https://doi.org/10.5194/bg-13-2913-2016
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.860214
Creator Rosas-Navarro, Anaid ORCID logo; Langer, Gerald ORCID logo; Ziveri, Patrizia ORCID logo
Publisher PANGAEA
Publication Year 2016
Funding Reference Seventh Framework Programme https://doi.org/10.13039/100011102 Crossref Funder ID 265103 https://cordis.europa.eu/project/id/265103 Mediterranean Sea Acidification in a Changing Climate
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 1130 data points
Discipline Earth System Research