Sugar permeability test in urine samples. 16 volunteers received treatments in different orders (Placebo, L. rhamnosus, Yogurt) and urine was collected after 2 and 5 hours. The samples were collected for the INSIDE explorative dietary study. The study was aimed to characterize the impact of fermented food may have on the small intestinal microbiota and systematically. Sucrose and Lactulose content in the urine was measured. Sucrose is hydrolyzed in the duodenum, and is therefore a good marker for gastroduodenal permeability, measured in 0-5h urine. Lactulose can permeate through the paracellular route and rhamnose through the transcellular route in the small intestine. The ratio in 0-5h urine is mainly used for general assessment of the SI permeability.

Date Submitted: 2021-10-27

The aim of the study is to determine the small intestinal microbiota response in humans to probiotic supplement interventions for two consecutive weeks, and to relate this to intestinal barrier function and immune and metabolic responses in blood, as functional outcome parameters of host physiology. A small cohort of ileostoma subjects has been recruited to participate in a three-arm intervention trial according to a double blind, cross-over, placebo-controlled design. Two probiotics products and a placebo were administered in random order to each volunteer for two weeks with 2 week wash-out periods in between interventions. The volunteers will participate in this study for approx. 100 days and will, in addition to the three interventions, not consume fermented products.