Supporting data for: Accelerated Sarcopenia Phenotype in the DJ-1/Park7-Knockout Zebrafish

DOI

This dataset represent muscle samples from 4 and 12 month- old wild type and DJ-1/PARK7 knockout zebrafish labeled with TMT16-plex.Eclipse Orbitrap instrument was used and the data analysed with Proteome Discoverer Version 2.5.

Abstract:Age-dependent loss of muscle mass and function is associated with oxidative stress. DJ-1/Park7 acts as an antioxidant through multiple signalling pathways. DJ-1-knockout zebrafish show a decline in swimming performance and loss of weight gain between 6 and 9 months of age. Here, we address the degree to which this is associated with muscle degeneration and identify molecular changes preceding dysregulation of muscle performance. Loss of DJ-1 reduced the skeletal muscle fibre cross-section area. The highly mitochondrial-dependent red slow muscle was more affected than the white muscle, and degeneration of sub-sarcolemma red muscle mitochondria was observed. Using TandemMassTag-based quantitative proteomics, we identified a total of 3721 proteins in the multiplex sample of 4 and 12-month-old muscles. A total of 68 proteins, mainly associated with inflammation and mitochondrial function, were dysregulated in the young DJ-1-null adults, with Annexin A3, Sphingomyelin phosphodiesterase acid-like 3B, Complement C3a, and 2,4-dienoyl CoA reductase 1 being the most affected. The loss of DJ-1 also accelerated molecular features associated with sarcopenia, such as a decrease in the NAD+/NADH ratio and a reduction in Prostaglandin reductase 2 and Cytosolic glycerol-3-phosphate dehydrogenase levels. In view of the experimental power of zebrafish, the DJ-1-null zebrafish makes a valuable model for understanding the connection between oxidative stress and age-dependent muscle loss and function.

Proteome Discoverer, 2.5

Identifier
DOI https://doi.org/10.18710/MLUU8I
Related Identifier IsCitedBy https://doi.org/10.3390/antiox13121509
Metadata Access https://dataverse.no/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.18710/MLUU8I
Provenance
Creator Rostad, Kristine O.; Trognitz, Tobias; Frøyset, Ann Kristin; Bifulco, Ersillia; Fladmark, Kari E. ORCID logo
Publisher DataverseNO
Contributor Fladmark, Kari E.; University of Bergen
Publication Year 2025
Funding Reference Advokat Rolf Sandberg Rebergs and Ellen Marie Rebergs Legat
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Fladmark, Kari E. (University of Bergen)
Representation
Resource Type experimental data; Dataset
Format text/plain; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Size 14898; 29747285; 19412150
Version 1.0
Discipline Life Sciences; Medicine
Spatial Coverage Bergen, Norway