Investigation of the translocation potential of therapeutic macromolecules into cell-derived exosomes


Exosomes are released by cells are responsible for cell-cell communication of macromolecules and the development of disease such as cancer. These nanoparticles are loaded with biologically active macromolecules, such interfering RNA and message RNA that mediate effects on the recipient cell. The discovery that Anthrax toxin can delivers toxins to neighbouring cells using exosome biogenesis has afforded an opportunity to develop a novel strategy for the investigation of this important pathological process, and to investigate a manufacturing process for the development of therapeutic exosomes for the treatment of common diseases using macromolecular therapies. We propose to investigate the ability of this toxin based system to package cell-derived exosomes with therapeutic macromolecules using neutron reflectometry to chart the translocation of these molecules in to the exosome.

Metadata Access
Creator Miss Rebecca Jones; Dr Paul Dyer; Dr Rob Barker; Dr Rebecca Welbourn; Miss Susan Shorter
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International;
OpenAccess true
Contact isisdata(at)
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2017-05-08T08:00:00Z
Temporal Coverage End 2017-05-12T07:50:31Z