Replication Data for:A small excitation window allows long-duration single-molecule imaging, with reduced background autofluorescence, in C. elegans neurons

DOI

The dataset contains the data and the MATLAB scripts associated with publication titled A small excitation window allows long-duration single-molecule imaging, with reduced background autofluorescence, in C. elegans neurons. Here we provide scripts and data corresponding to three different aspects: (1) the characterization of the technique, small-window illumination microscopy (SWIM), that was developed in this work (2) the numerical simulations to explain how SWIM reduces the amount of out-of-focus autofluoresence background during imaging. (3) the statistical analysis of the motion of vesicles moving back-and-forth in a C. elegans dendrite, imaged using SWIM.

The data and codes are orgainized in separate folders, with the titles indicating the figure the data belongs to. In addition, there are a couple of folders containing essential scripts used for analysis of single-molecule tracks. Each folder contains a ReadMe.txt file which details the data (and structure) and the accompanying script(s). Data is stored as .csv (or xlsx) files and scripts are written in MATLAB (The Math Works, Inc., R2021a).

Identifier
DOI https://doi.org/10.34894/DAJWJG
Related Identifier https://doi.org/10.1101/2022.12.20.521330
Metadata Access https://dataverse.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34894/DAJWJG
Provenance
Creator Mitra, Aniruddha ORCID logo; Elizaveta Loseva ORCID logo; Guus H. Haasnoot ORCID logo; E.J.G. Peterman (ORCID: 0000-0003-1058-249X)
Publisher DataverseNL
Contributor Mitra, Aniruddha
Publication Year 2023
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Mitra, Aniruddha (Vrije Universiteit Amsterdam)
Representation
Resource Type Dataset
Format text/csv; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet; text/x-matlab; text/plain; image/tiff
Size 71113; 2557; 4742; 373385; 202959; 7140; 237046; 2322; 2326; 2336; 2345; 2373; 953098; 596; 53845; 969; 109082; 67095; 46769; 65716; 2415; 527376; 893; 1117; 731; 1342; 8717; 1350; 8721; 1269; 8765; 1252; 8759; 1245; 8752; 5906; 1372; 3061; 4008; 3436; 6386; 15790; 3892; 1213; 1246; 1234; 2360; 1315; 1705; 2566; 1569; 1943; 1266; 1924; 8138; 5131; 868599; 1464200; 3465; 4246; 4448; 9543; 6381; 6038; 8368; 14707; 5066
Version 1.0
Discipline Life Sciences; Medicine; Natural Sciences; Physics