The dataset contains the data and the scripts (matlab and python) associated with the publication titled "DYF-5 regulates IFT by affecting train turnaround ". The data and codes are orgainized in separate folders, with the titles indicating the figure the data belongs to. In addition, there are a couple of folders containing essential scripts used for analysis of single-molecule tracks performed in the study. Each folder contains a ReadMe.txt file which details the data (and structure) and the accompanying script(s). Data is stored as .csv files.