Example multiplex immunofluorescence dataset for PUPAID analysis method

Dataset

DOI

This test/example dataset represents 17 contiguous TIFF-formatted tiles coming from an 8-plex immunofluorescence experiment that was performed on a human biopsy of minor salivary gland taken from a patient suffering from Sjögren’s syndrome. It is intended for learning and test purposes related to the PUPAID method, which is published in the PLOS One journal (see section “Relation Metadata”). The final staining panel used is composed of DAPI, anti-IL-21 (revealed with Opal 480), anti-CD21 (revealed with Opal 520), anti-TNFα (revealed with Opal 540), anti-CD4 (revealed with Opal 570), anti-IFNγ (revealed with Opal 620), anti-CD27 (revealed with Opal 650) and anti-CD20 (revealed with Opal 690). The full details about the used methodology are described in the provided “readme.txt” file.

The full details about the used methodology are described in the provided “readme.txt” file.

The minor salivary gland biopsy from which the dataset was generated was performed on 2017-03-09.

Identifier
DOI	https://doi.org/10.17026/LS/7XQFAT
Metadata Access	https://lifesciences.datastations.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.17026/LS/7XQFAT

Provenance
Creator	P. Régnier
Publisher	DANS Data Station Life Sciences
Contributor	Paul Régnier; Centre d'Histologie, Imagerie et Cytométrie (CHIC)
Publication Year	2024
Rights	CC BY 4.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/licenses/by/4.0
OpenAccess	true
Contact	Paul Régnier (Immunology-Immunopathology-Immunotherapy (i3) Laboratory, INSERM UMR-S 959, Sorbonne Université, Paris, France)

Representation
Resource Type	17 TIFF image files containing multiplex immunofluorescence-based data; Dataset
Format	image/tiff; text/plain
Size	80701786; 81600286; 82790002; 80921605; 81477018; 76117921; 76971999; 73478473; 81490539; 72412598; 72629553; 80499473; 78013341; 70146848; 79969745; 76421869; 71944093; 2675
Version	1.1
Discipline	Immunology; Life Sciences; Medicine; Microbiology, Virology and Immunology
Spatial Coverage	Centre de recherche des Cordeliers 15 rue de l’École de Médecine 75006 PARIS