Structure and activity of Membrane Proteins immobilised to solid supports.

DOI

Membrane Proteins (MPs) consist of about 1/3 of the proteome but the structural information available is very limited compared to that for soluble proteins. Despite this at least 60 % of all commercially available drugs are targeted at MPs. The insolubility of MPs in water has meant they have been more difficult to study than soluble proteins. One method of making them soluble in water while retaining functionality is to surround the hydrophobic section of the protein with an amphiphilic polymer, amphipol. Amphipols can be functionalised to bind to surfaces thereby tethering the MP to a platform. We (J-L. P) have achieved this and undertaken functional studies on such systems. We now aim to extend these studies to include the determination of the structural aspects of the immobilised MP layer and complexes. This will assist with optimisation of MP based device development.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24079120
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24079120
Provenance
Creator Dr Stephen Holt
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-12-07T09:07:31Z
Temporal Coverage End 2012-09-06T10:36:54Z