In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming

Here, using direct RNA sequencing and Illumina RNA sequencing, we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012B6131A59579B914FEF6D8317E32D58B489CCCB2E
Metadata Access https://data.blue-cloud.org/api/collections/B6131A59579B914FEF6D8317E32D58B489CCCB2E
Provenance
Instrument Illumina NovaSeq 6000; MinION; ILLUMINA; OXFORD_NANOPORE
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Coverage Begin 2022-02-10T00:00:00Z
Temporal Coverage End 2022-06-14T00:00:00Z