Series of three-dimensional fluorescence microscopy videos of the rat cardiomyoblast cell-line H9c2. Where indicated in the file name, the cells were adapted to galactose (galac). Normal growth conditions are with glucose (gluc). The outer mitochondrial membranes are labelled with the double tag mCherry-EGFP-OMP-25TM (dTag), i.e., both a red and green fluorescent protein variant. dTag can be used as a sensor for acidity (or lysosomal degradation), as the green fluorescence is lost sooner in an acidic environment than the red fluorescence. Where indicated, the samples are additionally labelled with LysoTracker Deep Red (LTDR).

This data is a collection of fluorescence microscopy videos acquired to study the dynamics of mitochondria and the degradation of mitochondria in lysosomes. Correlative transmission microscopy images (trans) were provided for machine learning to later enable label-free detection of mitochondria and lysosomes.

Each folder contains data from different experimental conditions:

mCh_eGFP_CCCP: mitochondrial outer membrane protein 25 is dually labelled using mCherry-EGFP. The time-lapses in this folder can be used to study the effect of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (see more details under methodological information.)

mCh_eGFP_LTDR: mitochondrial outer membrane protein 25 is dually labelled using mCherry-EGFP and acidic compartments using Lysotracker deep red.

mCh_eGFP_LTDR_trans: mitochondrial outer membrane protein 25 is dually labelled using mCherry-EGFP and acidic compartments using Lysotracker deep red. The fluorescence data has correlative label-free brightfield/transmission images (channel 4).

mCh_eGFP_trans: mitochondrial outer membrane protein 25 is dually labelled using mCherry-EGFP. The fluorescence data has correlative label-free brightfield/transmission images (channel 4).