Cuticle relative atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification

DOI

We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the relative atomic weight composition (weight %/atomic weight of element) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged. EDX was measured with with two machines (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 20 kV acceleration voltage. Spectra were taken on the cross-sectional surface of the exocuticle and the endocuticle layers of the carapace spine and antenna base and the core and outer ring of the horn tip.

Identifier
DOI https://doi.org/10.1594/PANGAEA.945338
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.945362
Related Identifier IsSupplementTo https://doi.org/10.3389/fmars.2022.909017
Related Identifier References https://doi.org/10.5281/zenodo.6596558
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.945338
Provenance
Creator Lowder, Kaitlyn ORCID logo; deVries, Maya S ORCID logo; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer
Publisher PANGAEA
Publication Year 2022
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 9733 data points
Discipline Earth System Research
Spatial Coverage (-117.269 LON, 32.853 LAT); off Southern California