The phenomenon of cooperation is prevalent in nature and found at all levels of life. In microbial communities, some groups of cells exhibit cooperative behaviour by producing costly extracellular resources that are freely available to others, and these resources are referred to as public goods. For cooperators to survive cheating and thrive in nature, they should have evolved some survival strategies. Therefore, to understand the adaptation of Saccharomyces cerevisiae cooperators in sucrose, we performed 200 generations of coevolution of cheaters and cooperators in sucrose to study how cooperators thrive in the presence of cheaters.