Peptidomimetics are synthetic mimics of peptides, the natural building blocks of proteins, and their development is a growing field of science. An underlying theme in recent peptidomimetic research is the connection between function and structure. For their potential to be fully exploited, a detailed understanding of their structure and interactions is required. In this proposal we will complete solution-based structural investigations of L-Alanine and its comparable analogues, β-alanine and the alanine-monopeptoid, sarcosine. We aim to answer the following questions:i)How do alanine, β¿alanine and sarcosine differ in structure in solution at the atomic level?ii)How are the three analogues hydrated?iii)What important monomer-monomer interactions are there for each analogue solution?These experiments will provide a structural benchmark for all our future studies.