Kelp holobiont responses to run-off in Billefjorden, Svalbard in August 2022


Kelps act as ecosystem engineers and foundation species, providing the food web basis for Arctic coastal, rocky shore ecosystems. Arctic fjords are undergoing large climatic changes, with the consequences of glacial and terrestrial run-off on kelp holobionts largely being unknown. We compared areas being influenced by either glacial or terrestrial run-off with a control area in Billefjorden, Svalbard in August 2022. Kelps were collected from three sampling stations (A-I) per area, with a plant rake (Plant rake 19.000, acc. to Sigurd Olsen, KC Denmark, Silkeborg, Denmark) on 5±2 m water depth. Silica-dried meristematic tissue was used for biochemical (pigment, antioxidant activity) analyses. The tissue above the meristem was rinsed with ultrapure water and freeze-dried, before elements were measured of homogenized, digested material with a triple quadrupole ICP-MS/MS system (Agilent 8800, Agilent Technologies; Tokyo, Japan). For microbial analyses, an area of 10×10 cm above the meristem was swabbed with a sterile cotton swab. The V4-V5 regions (515F: 5'-GTGCCAGCMGCCGCGGTAA-3' and 907R: 5'-CCGTCAATTCMTTTRAGTTT-3') of the bacterial 16S rRNA gene from DNA extracts were amplified and sequenced using the Illumina Nova platform (Shanghai Hanyu Biotech lab, Shanghai, China).

Metadata Access
Creator Niedzwiedz, Sarina ORCID logo; Schmidt, Claudia Elena; Yang, Yunlan; Burgunter-Delamare, Bertille; Andersen, Sebastian; Hildebrandt, Lars; Pröfrock, Daniel; Thomas, Helmuth ORCID logo; Zhang, Rui ORCID logo; Damsgård, Børge; Bischof, Kai ORCID logo
Publisher PANGAEA
Publication Year 2024
Funding Reference Horizon 2020 Crossref Funder ID 869383 ECOTIP; National Key Research and Development Program of China Crossref Funder ID 2021YFE0193000 ; Southern Marine Science and Engineering Guangdong Laboratory Crossref Funder ID SML2023SP218
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints);
OpenAccess false
Resource Type Dataset
Format text/tab-separated-values
Size 6004 data points
Discipline Basic Biological and Medical Research; Biochemistry; Biology; Life Sciences
Spatial Coverage (16.399W, 78.601S, 16.902E, 78.691N); Svalbard
Temporal Coverage Begin 2022-08-22T00:00:00Z
Temporal Coverage End 2022-08-29T00:00:00Z