Integrated abundance, cell volume, viability and leucine incorporation rates of prokaryotes in the epipelagic, mesopelagic and bathypelagic layers of the tropical and subtropical Atlantic

DOI

This dataset contains the results of the characterisation of the prokaryotic community by flow cytometry and tritiated leucine incorporation from the MAFIA cruise (Migrants and Active Flux In the Atlantic ocean). Samples were collected in the tropical and subtropical Atlantic during the MAFIA cruise (April 2015) on board the BIO Hespérides. Seawater samples were collected at 13 stations (from the Brazilian coast to the Canary Islands), from the surface down to 3500 m, using a General Oceanics oceanographic rosette equipped with 24 l PVC Niskin bottles. Abundance and cell characteristics (high nucleic acid content fraction, cell volume, viability) were based on measurements performed with a FACSCalibur (Becton-Dickinson) flow cytometer. Leucine incorporation rates were estimated with tritiated leucine (Kirchman et al. 1985) using centrifugation and filtration methods (Smith and Azam 1992). Integrated or average values of variables were estimated for the epipelagic (0-200 m), mesopelagic (200-1000 m) and bathypelagic (1000-3000 m) layers, based on an interpolated grid estimated with DIVA. The aim of this dataset was to estimate the influence of surface productivity on the standing stock, characteristics and activity (as leucine incorporation) of prokaryotes across the water column.

Identifier
DOI https://doi.org/10.1594/PANGAEA.943416
Related Identifier https://doi.org/10.1594/PANGAEA.943417
Related Identifier https://doi.org/10.1101/2022.06.20.496789
Related Identifier https://doi.org/10.1002/lno.12248
Related Identifier https://doi.org/10.3354/ame042159
Related Identifier https://doi.org/10.1128/AEM.01668-07
Related Identifier https://doi.org/10.20351/29HE20150403
Related Identifier https://doi.org/10.1128/aem.49.3.599-607.1985
Related Identifier https://doi.org/10.1128/AEM.67.4.1775-1782.2001
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.943416
Provenance
Creator Gómez-Letona, Markel ORCID logo; Arístegui, Javier ORCID logo; Hernández-Hernández, Nauzet ORCID logo; Pérez-Lorenzo, Maria; Alvarez-Salgado, Xose Anton; Teira, Eva ORCID logo; Sebastian, Marta ORCID logo
Publisher PANGAEA
Publication Year 2022
Funding Reference Horizon 2020 https://doi.org/10.13039/501100007601 Crossref Funder ID 817806 https://doi.org/10.3030/817806 Sustainable management of mesopelagic resources; Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 Crossref Funder ID CTM2012-39587-C04 Migrants and Active Flux In the Atlantic Ocean; Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 Crossref Funder ID CTM2015-69392-C3 Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump; Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 Crossref Funder ID CTM2017-83362-R INTERES: Papel de las interacciones fitoplancton-bacterias en la respuesta del plancton microbiano a la entrada de nutrientes alóctonos; Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 Crossref Funder ID PID2019-109084RB-C21 Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 375 data points
Discipline History; Humanities
Spatial Coverage (-33.959W, -13.124S, -15.946E, 27.404N); Atlantic Ocean; subtropical - tropical Atlantic
Temporal Coverage Begin 2015-04-05T00:45:00Z
Temporal Coverage End 2015-04-28T20:32:07Z