Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: genotyping


Measurements of genotype composition in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. The genotype composition was assessed for each species after batch cycles 7,13, and 26 by using microsatellites.

DOI https://doi.pangaea.de/10.1594/PANGAEA.957916
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.957930
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.957916
Creator Matthiessen, Birte ORCID logo; Hattich, Giannina S I ORCID logo; Pulina, Silvia ORCID logo; Hansen, Thomas; Reusch, Thorsten B H ORCID logo; Hamer, Jorin ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID MA5058/2-2 ; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID RE1708/17-2
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints); https://creativecommons.org/licenses/by/4.0/
OpenAccess false
Resource Type Dataset
Format text/tab-separated-values
Size 3852 data points
Discipline Earth System Research