This project aims to create a multi-locus genetic barcode reference library for marine species of interest to NOAA's National Marine Fisheries Science Centers. Each sample is voucher-based using curated and well identified specimens residing in museum collections. Reference sequences, e.g., annotated complete mitochondrial genomes and ribosomal repeats, will be derived by skimming from whole genome sequencing. These sequences will be critical for detecting marine species within environmental samples, many of which are being taken by different practitioners with varying aims.