Exploring the interactions between vesicles/viruses and supported lipid bilayers using neutron reflectometry

DOI

Many biological processes occur at the interface of cells, at the so called cell membrane, such as internalization of biological nanoparticles, including virions, exosomes and nanoparticle-based drug-delivery vehicles. In this project, we plan to use neutron reflectometry (NR) to measure the distances between a supported cell-membrane mimic and different models of lipid-based biological nanoparticles. In this way, we will assess how the distance between these entities is influenced by external components, e.g. charge and ligand-receptor density, to thereby gain new knowledge on how e.g. binding-induced nanoparticle deformation and membrane fusion are controlled by inter-membrane distance. This project should be the first of its kind to address such questions using NR, and will contribute improved determination of inter-membrane distances in biologically important processes.

Identifier
DOI https://doi.org/10.5286/ISIS.E.86391220
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/86391220
Provenance
Creator Dr Hudson Pace; Dr Rebecca Welbourn; Dr Antonius Armanious; Dr Yuri Gerelli; Dr Emanuel Schneck; Dr Samantha Micciulla; Professor Fredrik Höök; Dr Bjorn Agnarsson
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences; Physics
Temporal Coverage Begin 2017-05-18T08:00:00Z
Temporal Coverage End 2017-05-22T08:00:00Z