Increased environmental pressures and awareness is promoting a major drive towards an increased use of biosurfactants to produce more biosustainable and biodegradable consumer products, in home and personal care products, cosmetics and foodstuffs. One area of promise is the use of the highly surface active hydrophobin, and its incorporation with other proteins such as β-casein and food grade polysorbate surfactants such as polyoxyethylene sorbitan monolaurate. The focus of this proposal is to investigate the self-assembly of hydrophobin with the family of different polysorbate surfactants.