Microhaplotype genotyping-by-sequencing of 98 highly polymorphic markers in three chestnut tree species

DOI

Chestnut species have large ecological, cultural and economic importance. Developing genetic markers for these species is of interest for conservation, breeding or evolutionary studies. We designed 192 primer pairs targeting microsatellites detected in the Castanea mollissima reference genome and tested them on C. sativa and C. crenata. We PCR amplified 3×50 microsatellites in 109 chestnut trees. Microhaplotype calling accounting for all polymorphisms resulted in a total of 98 high confidence polymorphic markers.

Identifier
DOI https://doi.org/10.15454/PNBEAM
Related Identifier https://doi.org/10.1007/s12686-020-01157-5
Metadata Access https://entrepot.recherche.data.gouv.fr/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.15454/PNBEAM
Provenance
Creator Laurent, Benoit (ORCID: 0000-0001-5042-933X)
Publisher Recherche Data Gouv
Contributor Laurent, Benoit
Publication Year 2020
Funding Reference Agence Nationale Recherche ANR-10-EQPX-16 ; Region Nouvelle Aquitaine 2018-1R20204
Rights etalab 2.0; info:eu-repo/semantics/openAccess; https://spdx.org/licenses/etalab-2.0.html
OpenAccess true
Contact Laurent, Benoit (INRA - Institut National de la Recherche Agronomique)
Representation
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Version 5.1
Discipline Agriculture, Forestry, Horticulture; Geosciences; Life Sciences; Plant Science; Silviculture; Ecology