Solid-supported lipid bilayers for studying mechanosensitive ion channels: to tether or not

DOI

Bacterial membranes contain safety valves called mechanosensitive ion channels, which protect the bacterium from changes in the osmotic pressure of their environment. However, they also represent a possible achilles heal, which we may be able to exploit to help overcome the current rise in bacterial antimicrobial resistance. The ion channels could be actuated by a change in membrane tension caused by therapeutic molecules interacting with the membrane. We will develop a platform in which the ion channel is embedded in a model bacterial bilayer and either specifically tethered or not tethered at the surface. We will use the reflection of neutrons to probe if the addition of the therapeutic peptide causes a change in the curvature/tension of the bilayer in which the ion channels have been embedded and in doing so causes the channel to open, increasing the amount of water in the bilayer.

Identifier
DOI https://doi.org/10.5286/ISIS.E.95664614
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/95664614
Provenance
Creator Dr Sophie Ayscough; Dr Simon Titmuss; Dr Maxmilian Skoda
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences; Physics
Temporal Coverage Begin 2018-06-25T08:00:00Z
Temporal Coverage End 2018-06-27T15:57:02Z