Protein interactions at solid-liquid interfaces: The effect of affinity columns on protein structure

DOI

Biopharmaceuticals are manufactured using chromatographic procedures including adsorption and desorption processes at the solid-liquid interface. These events take place in porous particles packed within a column. The separation is carried out under plug flow conditions, which allows the high surface area within the particles to be utilised. The traditional indirect method to measure intra-particle pore structure lacks the resolution to identify how proteins bind to these complex structures. In this application, we wish to extend initial proof of principal work which has demonstrated the use of SANS, to uncover the heterogeneous pore distribution and the sequence in which they are occupied during a chromatographic process. Our experimental design will enable us to model this and to measure the resin structure before and post adsorption with the therapeutic antibody.

Identifier
DOI https://doi.org/10.5286/ISIS.E.95670476
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/95670476
Provenance
Creator Professor Christopher Roberts; Dr James Doutch; Miss Maria Papachristodoulou; Dr Paul Butler; Dr Luke Clifton; Miss Hoi Sang Beatrice Leung; Professor Daniel Bracewell
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Construction Engineering and Architecture; Engineering; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-06-06T08:00:00Z
Temporal Coverage End 2018-06-08T08:04:17Z