Analysis of horizontal gene transfer (HGT) candidates on the M. incognita predicted proteins from (Blanc-Mathieu et al. 2017), using Alienness (Rancurel et al. 2017) on a Diamond search against the NCBI's nr library. The following parameters were used in Alienness Taxonomic group of interest: 33208 (Metazoa; HGT of non metazoan origin in a metazoan species) Taxonomic group to exclude: 33283 (Tylenchomorpha; to ignore self hits to plant-parasitic nematodes in Clade 12) Additional taxonomic groups to classify potential donors: 3041 (Chlorophyta); 35493 (Streptophyta) M. incognita proteins with an AI>0 have a better hit to non-metazoa than metazoa and this can include possible HGT M. incognita proteins with an AI>14 have a better hit to non-metazoa than metazoa, with a magnitude of difference >1E6 and this can be considered likely HGT